Lot-to-Lot Variation in Adeno-Associated Virus Serotype 9 (AAV9) Preparations

Biodistribution of adeno-associated virus serotype 9 (AAV9) vector after intrathecal and intravenous delivery in mouse

Adeno-associated virus serotype 9 (AAV9)-mediated gene transfer has been reported in central nervous system (CNS) and peripheral tissues. The current study compared the pattern of expression of Green Fluorescent Protein (GFP) across the mouse CNS and selected peripheral tissues after intrathecal (i.t.) or intravenous (i.v.) delivery of equivalent doses of single-stranded AAV9 vector. After i.t....

Lot-to-lot variation in anti-hepatitis C signal-to-cutoff ratio.

Lot-to-Lot Variation in Anti-Hepatitis C Signal-to-Cutoff Ratio, D. Robert Dufour (Pathology and Laboratory Medicine Service, Veterans Affairs Medical Center, and Department of Pathology, The George Washington University Medical Center, Washington, DC; address for correspondence: Pathology and Laboratory Medicine Service-113, Veterans Affairs Medical Center, 50 Irving St. NW, Washington DC 2042...

Structure of adeno-associated virus serotype 5.

Adeno-associated virus serotype 5 (AAV5) requires sialic acid on host cells to bind and infect. Other parvoviruses, including Aleutian mink disease parvovirus (ADV), canine parvovirus (CPV), minute virus of mice, and bovine parvovirus, also bind sialic acid. Hence, structural homology may explain this functional homology. The amino acids required for CPV sialic acid binding map to a site at the...

Recombinant adeno-associated virus serotype 9 leads to preferential cardiac transduction in vivo.

Heart disease is often the end result of inherited genetic defects, which may potentially be treatable using a gene-transfer approach. Recombinant adeno-associated virus (rAAV)-mediated gene delivery has emerged as a realistic method for the treatment of such disorders. Here, we demonstrate and compare the natural affinity of specific AAV serotype capsids for transduction of cardiac tissue. We ...

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